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Virol J. 2018 Mar 20;15(1):49. doi: 10.1186/s12985-018-0955-1.

Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector.

Xu H1,2, Bao X1, Wang Y1, Xu Y1, Deng B1,2, Lu Y1,2, Hou J3,4.

Author information

1
Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu Province, 210014, China.
2
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu province, 225009, China.
3
Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu Province, 210014, China. houjiboccvv@163.com.
4
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu province, 225009, China. houjiboccvv@163.com.

Abstract

BACKGROUND:

DNA delivery with bacteriophage by surface-displayed mammalian cell penetrating peptides has been reported. Although, various phages have been used to facilitate DNA transfer by surface displaying the protein transduction domain of human immunodeficiency virus type 1 Tat protein (Tat peptide), no similar study has been conducted using T7 phage.

METHODS:

In this study, we engineeredT7 phage as a DNA targeting delivery vector to facilitate cellular internalization. We constructed recombinant T7 phages that displayed Tat peptide on their surface and carried eukaryotic expression box (EEB) as a part of their genomes (T7-EEB-Tat).

RESULTS:

We demonstrated that T7 phage harboring foreign gene insertion had packaged into infective progeny phage particles. Moreover, when mammalian cells that were briefly exposed to T7-EEB-Tat, expressed a significant higher level of the marker gene with the control cells infected with the wide type phage without displaying Tat peptides.

CONCLUSION:

These data suggested that the potential of T7 phage as an effective delivery vector for DNA vaccine transfer.

KEYWORDS:

Bacteriophage T7; Eukaryotic expression; Targeting delivery; Vaccine

PMID:
29558962
PMCID:
PMC5859711
DOI:
10.1186/s12985-018-0955-1
[Indexed for MEDLINE]
Free PMC Article

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