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Protein Cell. 2019 Jan;10(1):43-59. doi: 10.1007/s13238-018-0512-0. Epub 2018 Mar 20.

Neoblast-enriched zinc finger protein FIR1 triggers local proliferation during planarian regeneration.

Author information

1
Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine & Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200025, China.
2
Department of Cardiology, Changhai Hospital, Shanghai, 200433, China.
3
Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine & Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200025, China. qjing@sibs.ac.cn.
4
Department of Cardiology, Changhai Hospital, Shanghai, 200433, China. qjing@sibs.ac.cn.

Abstract

Regeneration, relying mainly on resident adult stem cells, is widespread. However, the mechanism by which stem cells initiate proliferation during this process in vivo is unclear. Using planarian as a model, we screened 46 transcripts showing potential function in the regulation of local stem cell proliferation following 48 h regeneration. By analyzing the regeneration defects and the mitotic activity of animals under administration of RNA interference (RNAi), we identified factor for initiating regeneration 1 (Fir1) required for local proliferation. Our findings reveal that Fir1, enriched in neoblasts, promotes planarian regeneration in any tissue-missing context. Further, we demonstrate that DIS3 like 3'-5' exoribonuclease 2 (Dis3l2) is required for Fir1 phenotype. Besides, RNAi knockdown of Fir1 causes a decrease of neoblast wound response genes following amputation. These findings suggest that Fir1 recognizes regenerative signals and promotes DIS3L2 proteins to trigger neoblast proliferation following amputation and provide a mechanism critical for stem cell response to injury.

KEYWORDS:

Dis3l2; Schmidtea mediterranea; adult stem cells; local proliferation; planarians; wound recognition

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