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Sci Rep. 2018 Mar 19;8(1):4852. doi: 10.1038/s41598-018-23034-w.

ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease.

Author information

1
Department of Clinical Sciences, Swedish University of Agricultural Sciences, SE-750 07, Uppsala, Sweden. hanna.bremer@slu.se.
2
Department of Medicine Solna, CMM, L8:01, Karolinska University Hospital, Karolinska Institutet, SE-171 76, Stockholm, Sweden.
3
Science for Life Laboratory, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
4
Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH Royal Institute of Technology, SE-171 21, Solna, Sweden.
5
Euroimmun AG, 23560, Lübeck, Germany.
6
Departement of Medical Sciences, Rheumatology and Science for Life Laboratory, Uppsala University, SE 751 85, Uppsala, Sweden.
7
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, SE-750 07, Uppsala, Sweden.
8
Department of Clinical Sciences, Swedish University of Agricultural Sciences, SE-750 07, Uppsala, Sweden.
9
Broad Institute of Harvard and MIT, Cambridge, MA, 02142, USA.
10
Science for Life Laboratory, IMBIM, Uppsala University, SE-751 23, Uppsala, Sweden.
11
Department of Clinical Science and K.G. Jebsen Center for Autoimmune disorders, University of Bergen, 5021, Bergen, Norway.
12
Department of Medicine, Haukeland University Hospital, 5021, Bergen, Norway.

Abstract

Dogs can spontaneously develop complex systemic autoimmune disorders, with similarities to human autoimmune disease. Autoantibodies directed at self-antigens are a key feature of these autoimmune diseases. Here we report the identification of interleukin enhancer-binding factors 2 and 3 (ILF2 and ILF3) as autoantigens in canine immune-mediated rheumatic disease. The ILF2 autoantibodies were discovered in a small, selected canine cohort through the use of human protein arrays; a method not previously described in dogs. Subsequently, ILF3 autoantibodies were also identified in the same cohort. The results were validated with an independent method in a larger cohort of dogs. ILF2 and ILF3 autoantibodies were found exclusively, and at a high frequency, in dogs that showed a speckled pattern of antinuclear antibodies on immunofluorescence. ILF2 and ILF3 autoantibodies were also found at low frequency in human patients with SLE and Sjögren's syndrome. These autoantibodies have the potential to be used as diagnostic biomarkers for canine, and possibly also human, autoimmune disease.

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