Format

Send to

Choose Destination
Biomaterials. 2018 Jun;167:69-79. doi: 10.1016/j.biomaterials.2018.03.019. Epub 2018 Mar 13.

Transcutaneous implantation of valproic acid-encapsulated dissolving microneedles induces hair regrowth.

Author information

1
Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea.
2
Translational Research Center for Protein Function Control, Building 117, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea.
3
Department of Biotechnology, Building 123, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea; Juvic Biotech, Inc., Building 102, Yonsei Engineering Research Park, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea. Electronic address: hijung@yonsei.ac.kr.

Abstract

The interest in alternative material systems and delivery methods for treatment of androgenetic alopecia has been increasing in the recent decades. Topical application of valproic acid (VPA), an FDA-approved anticonvulsant drug, has been shown to effectively stimulate hair follicle (HF) regrowth by upregulating Wnt/β-catenin, a key pathway involved in initiation of HF development. Moreover, a majority of studies have suggested that cutaneous wound re-epithelialization is capable of inducing HF through Wnt/β-catenin pathway. Here, we report fabrication and evaluation of a novel VPA-encapsulating dissolving microneedle (DMN-VPA) that creates minimally invasive dermal micro-wounds upon application, significantly improving the VPA delivery efficiency. DMN-VPA not only delivers encapsulated VPA with higher accuracy than topical application, it also stimulates wound re-epithelialization signals involved in HF regrowth. Through a series of in vivo studies, we show that micro-wounding-mediated implantation of DMN-VPA upregulates expression of Wnt/β-catenin pathway, alkaline phosphatase, proliferating cell nuclear antigen, loricrin and HF stem cell markers, including keratin 15, and CD34 more effectively than topical application.

KEYWORDS:

Androgenetic alopecia; Dissolving microneedle; Hair regrowth; Micro-wounding; Transdermal drug delivery; Valproic acid

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center