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Microb Pathog. 2018 May;118:140-145. doi: 10.1016/j.micpath.2018.03.026. Epub 2018 Mar 16.

Antimicrobial susceptibility and characterization of metallo-β-lactamases, extended-spectrum β-lactamases, and carbapenemases of Bacillus cereus isolates.

Author information

1
Faculty of Health Sciences, University of Ljubljana, Zdravstvena Pot 5, SI-1000, Ljubljana, Slovenia. Electronic address: karmen.torkar@zf.uni-lj.si.
2
Clinical Department of Clinical and Molecular Microbiology, School of Medicine, University Hospital Center Zagreb, Kispatic Street 12, HR-10000, Zagreb, Croatia.

Abstract

The susceptibility of 66 clinical and environmental B. cereus isolates were tested to selected antimicrobials by a broth microdilution method. All strains were resistant to β-lactams and susceptible to gentamicin and imipenem. Sixty-five (98.5%) isolates were susceptible to meropenem and ciprofloxacin and 74.2% to azithromycin. Significant differences in MIC values between environmental and clinical isolates were not demonstrated (p > 0.05). According to the disc diffusion method, 80.3%-98.5% of the strains were resistant to one or more of four cephalosporins. The presence of genes for B. cereus β-lactamases BCI, BCII, BCIII, extended-spectrum β-lactamases from the CTX and TEM family and the carbapenemases belonging to IMP and VIM family was studied. BlaII genes were expressed in all isolates; the PCR products for blaIII were also detected in two strains, but none of them was positive for blaI. The amplicon of the family blaCTX-M, mostly M-1 and M-15, was confirmed among 68.2% of the isolates, while were blaVIM-like genes determined in 21.2% of the samples.

KEYWORDS:

Bacillus cereus; Carbapenemases; Extended–spectrum β-lactamases; Susceptibility; β-lactamases

PMID:
29551437
DOI:
10.1016/j.micpath.2018.03.026
[Indexed for MEDLINE]

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