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Stem Cell Res. 2018 Apr;28:177-180. doi: 10.1016/j.scr.2018.02.007. Epub 2018 Feb 18.

Generation of a transgene-free human induced pluripotent stem cell line (UNIPDi001-A) from oral mucosa epithelial stem cells.

Author information

1
Department of Molecular Medicine, University of Padova, 35121 Padua, Italy.
2
Department of Molecular Medicine, University of Padova, 35121 Padua, Italy. Electronic address: marta.trevisan@unipd.it.
3
Fondazione Banca degli Occhi del Veneto, 30174 Venice, Italy.

Abstract

Human oral mucosa epithelial stem cells (hOMESCs) were obtained from a fresh oral biopsy collected from a healthy subject at the Fondazione Banca degli Occhi del Veneto (FBOV). An integration-free reprogramming protocol was applied exploiting episomal plasmids transfected into cells using a Nucleofector device. Around day 20 post transfection, several human induced pluripotent stem cell (hiPSC) colonies were manually picked and expanded. One of these (UNIPDi001-A-hiPSCs) expressed undifferentiated state marker alkaline phosphatase along with a panel of pluripotency state markers and was able to differentiate into the derivatives of all the three germ layers.

PMID:
29547871
DOI:
10.1016/j.scr.2018.02.007
[Indexed for MEDLINE]
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