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PLoS One. 2018 Mar 15;13(3):e0194063. doi: 10.1371/journal.pone.0194063. eCollection 2018.

A modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings.

Author information

1
Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui, China.
2
School of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, China.

Abstract

In this paper we report the development of a cost-effective, modular, open source, and fully automated slide-scanning microscope, composed entirely of easily available off-the-shelf parts, and capable of bright field and fluorescence modes. The automated X-Y stage is composed of two low-cost micrometer stages coupled to stepper motors operated in open-loop mode. The microscope is composed of a low-cost CMOS sensor and low-cost board lenses placed in a 4f configuration. The system has approximately 1 micron resolution, limited by the f/# of available board lenses. The microscope is compact, measuring just 25×25×30 cm, and has an absolute positioning accuracy of ±1 μm in the X and Y directions. A Z-stage enables autofocusing and imaging over large fields of view even on non-planar samples, and custom software enables automatic determination of sample boundaries and image mosaicking. We demonstrate the utility of our device through imaging of fluorescent- and transmission-dye stained blood and fecal smears containing human and animal parasites, as well as several prepared tissue samples. These results demonstrate image quality comparable to high-end commercial microscopes at a cost of less than US$400 for a bright-field system, with an extra US$100 needed for the fluorescence module.

PMID:
29543835
PMCID:
PMC5854341
DOI:
10.1371/journal.pone.0194063
[Indexed for MEDLINE]
Free PMC Article

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