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Sci Rep. 2018 Mar 14;8(1):4491. doi: 10.1038/s41598-018-22826-4.

Genetic variations in the fusion protein of respiratory syncytial virus isolated from children hospitalized with community-acquired pneumonia in China.

Chen X1,2, Xu B2, Guo J1,2, Li C3, An S4, Zhou Y5, Chen A6, Deng L7, Fu Z8, Zhu Y1,2, Liu C1,2, Xu L1,2, Wang W1,2, Shen K9, Xie Z10,11.

Author information

1
Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Beijing Pediatric Research Institute, Beijing, 100045, China.
2
Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 10045, China.
3
The 2nd Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
4
Children's Hospital of Hebei Province, Shijiazhuang, China.
5
The Children's Hospital of Zhejiang University School of Medicine, Hangzhou, China.
6
The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
7
Guangzhou Women and Children's Medical Center, Guangzhou, China.
8
Children's Hospital of Chongqing Medical University, Chongqing, China.
9
Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 10045, China. kunlingshen1717@163.com.
10
Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Beijing Pediatric Research Institute, Beijing, 100045, China. xiezhengde@bch.com.cn.
11
Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 10045, China. xiezhengde@bch.com.cn.

Abstract

To identify the variations in fusion (F) protein gene of RSV in China, a molecular epidemiological study was conducted. A total of 553 RSV positive specimens were collected from 2338 pediatric patients hospitalized with community-acquired pneumonia during a multi-center study conducted during 2014-2016. A total of 252 samples (183 RSV A, 69 RSV B) were selected for F gene sequencing, and analyzed together with 142 F gene sequences downloaded from GenBank. The result showed that all the Chinese RSV A and RSV B strains could be divided respectively into three branches. Compared with RSV A/B prototype sequences respectively, there were significant amino acid (AA) mutations at multiple antigenic sites. For RSV A, changes were found at AA residues 122, 124, 125, 276 and 384, and for RSV B at AA residues 45, 116, 125, 172, 173 and 202. Variations in human histocompatibility leukocyte antigen-restricted CTL epitopes were also observed. In total, 56 amino acid differences for the complete F protein were found between the RSV A and B groups in China, while several mutations were only found in the RSV B strains during 2015-2016. The RSV F gene is relatively conserved in China, however, limited mutations are still occurring with time.

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