Send to

Choose Destination
Front Cell Infect Microbiol. 2018 Feb 27;8:39. doi: 10.3389/fcimb.2018.00039. eCollection 2018.

Transcriptome Analysis of Pseudomonas aeruginosa Cultured in Human Burn Wound Exudates.

Author information

Microbiology Unit, Department of Botany and Plant Biology, Sciences III, University of Geneva, Geneva, Switzerland.
Plastic, Reconstructive and Hand Surgery, Unit of Regenerative Therapy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Department of Intensive Care Medicine, Bern University Hospital, Bern, Switzerland.
School of Pharmaceutical Sciences, University of Geneva and Centre Hospitalier Universitaire Vaudois, Geneva, Switzerland.


Pseudomonas aeruginosa is a severe opportunistic pathogen and is one of the major causes of hard to treat burn wound infections. Herein we have used an RNA-seq transcriptomic approach to study the behavior of P. aeruginosa PAO1 growing directly on human burn wound exudate. A chemical analysis of compounds used by this bacterium, coupled with kinetics expression of central genes has allowed us to obtain a global view of P. aeruginosa physiological and metabolic changes occurring while growing on human burn wound exudate. In addition to the numerous virulence factors and their secretion systems, we have found that all iron acquisition mechanisms were overexpressed. Deletion and complementation with pyoverdine demonstrated that iron availability was a major limiting factor in burn wound exudate. The quorum sensing systems, known to be important for the virulence of P. aeruginosa, although moderately induced, were activated even at low cell density. Analysis of bacterial metabolism emphasized importance of lactate, lipid and collagen degradation pathways. Overall, this work allowed to designate, for the first time, a global view of P. aeruginosa characteristics while growing in human burn wound exudate and highlight the possible therapeutic approaches to combat P. aeruginosa burn wound infections.


Pseudomonas aeruginosa; human burn wound exudates; infection; transcriptome; virulence factors

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center