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Sci Rep. 2018 Mar 13;8(1):4415. doi: 10.1038/s41598-018-22798-5.

Transcriptomic analysis of transgressive segregants revealed the central role of photosynthetic capacity and efficiency in biomass accumulation in sugarcane.

Author information

1
Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, Dallas, TX, 75252, USA.
2
Hawaii Agriculture Research Center, Kunia, HI, 96759, USA.
3
Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
4
Texas A&M AgriLife Research Center at Weslaco, Texas A&M University System, Weslaco, TX, 78596, USA.
5
Center for Genomics and Biotechnology, Fujian Provincial Key laboratory of Haixia applied plant systems biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China.
6
Texas A&M AgriLife Research Center at Dallas, Texas A&M University System, Dallas, TX, 75252, USA. qyu@ag.tamu.edu.
7
Center for Genomics and Biotechnology, Fujian Provincial Key laboratory of Haixia applied plant systems biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China. qyu@ag.tamu.edu.
8
Department of Plant Pathology & Microbiology, Texas A&M University, College Station, TX, 77843, USA. qyu@ag.tamu.edu.

Abstract

Sugarcane is among the most efficient crops in converting solar energy into chemical energy. However, due to its complex genome structure and inheritance, the genetic and molecular basis of biomass yield in sugarcane is still largely unknown. We created an F2 segregating population by crossing S. officinarum and S. spontaneum and evaluated the biomass yield of the F2 individuals. The F2 individuals exhibited clear transgressive segregation in biomass yield. We sequenced transcriptomes of source and sink tissues from 12 selected extreme segregants to explore the molecular basis of high biomass yield for future breeding of high-yielding energy canes. Among the 103,664 assembled unigenes, 10,115 and 728 showed significant differential expression patterns between the two extreme segregating groups in the top visible dewlap leaf and the 9th culm internode, respectively. The most enriched functional categories were photosynthesis and fermentation in the high-biomass and the low-biomass groups, respectively. Our results revealed that high-biomass yield was mainly determined by assimilation of carbon in source tissues. The high-level expression of fermentative genes in the low-biomass group was likely induced by their low-energy status. Group-specific expression alleles which can be applied in the development of new high-yielding energy cane varieties via molecular breeding were identified.

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