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Int J Mol Sci. 2018 Mar 10;19(3). pii: E800. doi: 10.3390/ijms19030800.

Astragalus membranaceus Extract Attenuates Inflammation and Oxidative Stress in Intestinal Epithelial Cells via NF-κB Activation and Nrf2 Response.

Author information

1
Department of Pharmacy, University of Salerno-Via Giovanni Paolo II, 132-84084 Fisciano-Salerno, Italy. sadesso@unisa.it.
2
Giellepi S.p.A. Health Science Department, Via Benvenuto Cellini 37, 20851 Lissone (Monza Brianza), Italy. rosario.russo@giellepi.it.
3
Department of Biomedical Sciences, Cornell University, Veterinary Research Tower, Cornell University, Ithaca, NY 14853-6401, USA. aq10@cornell.edu.
4
Department of Pharmacy, University of Salerno-Via Giovanni Paolo II, 132-84084 Fisciano-Salerno, Italy. autore@unisa.it.
5
Department of Pharmacy, University of Salerno-Via Giovanni Paolo II, 132-84084 Fisciano-Salerno, Italy. smarzocco@unisa.it.

Abstract

Astragalus membranaceus, dried root extract, also known as Astragali radix, is used in traditional Chinese medicine as a tonic remedy. Moreover, it has been reported that Astragalus membranaceus could attenuate intestinal inflammation; however, the underlying mechanism for its anti-inflammatory activity in intestinal epithelial cells (IECs) remains unclear. In this study, we evaluated Astragalus membranaceus extract (5-100 µg/mL) in a model of inflammation and oxidative stress for IECs. We showed that Astragalus membranaceus extract reduced the inflammatory response induced by lipopolysaccharide from E. coli (LPS) plus interferon-γ (IFN), decreasing tumor necrosis factor-α (TNF-α) release, cycloxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, nitrotyrosine formation, nuclear factor-κB (NF-κB) activation, and reactive oxygen species (ROS) release in the non-tumorigenic intestinal epithelial cell line (IEC-6). The antioxidant potential of Astragalus membranaceus extract was also evaluated in a model of hydrogen peroxide (H₂O₂)-induced oxidative stress in IEC-6, indicating that this extract reduced ROS release and increased nuclear factor (erythroid-derived 2)-like 2 (Nrf2) activation and the expression of antioxidant cytoprotective factors in these cells. The results contributed to clarify the mechanisms involved in Astragalus membranaceus extract-reduced inflammation and highlighted the potential use of this extract as an anti-inflammatory and antioxidant remedy for intestinal diseases.

KEYWORDS:

Astragalus membranaceus; inflammation; intestinal epithelial cells; oxidative stress

PMID:
29534459
PMCID:
PMC5877661
DOI:
10.3390/ijms19030800
[Indexed for MEDLINE]
Free PMC Article

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