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Gut Microbes. 2018 Jul 4;9(4):357-368. doi: 10.1080/19490976.2018.1445956. Epub 2018 Apr 27.

FUT2 genotype and secretory status are not associated with fecal microbial composition and inferred function in healthy subjects.

Author information

1
a Zane Cohen Centre for Digestive Diseases, Mount Sinai Hospital , Toronto , ON , Canada.
2
b Division of Gastroenterology, Department of Medicine , University of Toronto , Toronto , ON , Canada.
3
c Lunenfeld-Tanenbaum Research Institute, Sinai Health System , Toronto , Ontario , Canada.
4
d Division of Biostatistics, Dalla Lana School of Public Health , University of Toronto , Toronto , Ontario , Canada.
5
e Department of Cell & Systems Biology , University of Toronto , Toronto , Ontario , Canada.
6
f Centre for the Analysis of Genome Evolution & Function, University of Toronto , Toronto , Ontario , Canada.
7
g Division of Gastroenterology, Hepatology and Nutrition, Department of Paediatrics , The Hospital for Sick Children , Toronto , Ontario , Canada.
8
h Department of Medicine , McMaster University , Hamilton , Ontario , Canada.
9
i Inflammatory Bowel Disease Clinic, Division of Gastroenterology and Hepatology of Gastroenterology, University of Calgary , Calgary , Alberta , Canada.
10
j Department of Pediatrics , University of Alberta , Edmonton , Alberta , Canada.
11
k Montreal University, Hôpital Maisonneuve-Rosemont , Department of Medicine , Montreal , Quebec , Canada.
12
l Division of Gastroenterology and CEGIIR, Department of Medicine , University of Alberta , Edmonton , Alberta , Canada.
13
m Shaare Zedek Medical Center , Department of pediatric GI , Jerusalem , Israel.
14
n Division of Epidemiology, Dalla Lana School of Public Health , University of Toronto , Toronto , Ontario , Canada.
15
o Genetics and Genome Biology, The Hospital for Sick Children Research Institute, The Hospital for Sick Children , Toronto , Ontario , Canada.

Abstract

Heritability analysis of the microbiota has demonstrated the importance of host genotype in defining the human microbiota. The alpha (1,2)-fucosyltransferase 2 encoded by FUT2 is involved in the formation of the H antigen and the SNP, rs601338 is associated with ABO histo-blood group antigen secretion in the intestinal mucosa. Previous studies have provided non replicated results for the association of this polymorphism with the composition and inferred function of intestinal microbiota. We aimed to assess this relationship in a large cohort of 1,190 healthy individuals. Genotyping was performed using the HumanCoreEXOME chip, microbial composition was addressed by 16S rRNA gene sequencing. Firmicutes, Bacteroidetes, and Actinobacteria were the dominant phyla in this cohort. Although we have sufficient power to detect significant associations of FUT2 genotype/ inferred phenotype with the microbiota, our data demonstrate that FUT2 genotype and secretor status is not associated with microbial alpha diversity, microbial composition or inferred microbial function after correction for multiple testing. Thus, FUT2 genotype and inferred phenotype are not associated with human fecal microbial composition and imputed function.

KEYWORDS:

ABO antigen; Blood group antigens; Fecal microbiota; Fucosylation; Gut microbiota; Healthy human; Microbiome; human genetic; mucus

PMID:
29533703
PMCID:
PMC6219652
DOI:
10.1080/19490976.2018.1445956
[Indexed for MEDLINE]
Free PMC Article

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