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Sci Data. 2018 Mar 13;5:180036. doi: 10.1038/sdata.2018.36.

Global quantitative analysis of the human brain proteome in Alzheimer's and Parkinson's Disease.

Ping L1,2, Duong DM1,2, Yin L1,2, Gearing M2,3, Lah JJ2,4, Levey AI2,4, Seyfried NT1,2,4.

Author information

1
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
2
Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA.
3
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.
4
Department of Neurology, Emory University School of Medicine, Atlanta, GA 30322, USA.

Abstract

Patients with Alzheimer's disease (AD) and Parkinson's disease (PD) often have overlap in clinical presentation and brain neuropathology suggesting that these two diseases share common underlying mechanisms. Currently, the molecular pathways linking AD and PD are incompletely understood. Utilizing Tandem Mass Tag (TMT) isobaric labeling and synchronous precursor selection-based MS3 (SPS-MS3) mass spectrometry, we performed an unbiased quantitative proteomic analysis of post-mortem human brain tissues (n=80) from four different groups defined as controls, AD, PD, and co-morbid AD/PD cases across two brain regions (frontal cortex and anterior cingulate gyrus). In total, we identified 11 840 protein groups representing 10 230 gene symbols, which map to ~65% of the protein coding genes in brain. The utility of including two reference standards in each TMT 10-plex assay to assess intra- and inter-batch variance is also described. Ultimately, this comprehensive human brain proteomic dataset serves as a valuable resource for various research endeavors including, but not limited to, the identification of disease-specific protein signatures and molecular pathways that are common in AD and PD.

PMID:
29533394
PMCID:
PMC5848788
DOI:
10.1038/sdata.2018.36
[Indexed for MEDLINE]
Free PMC Article

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