Format

Send to

Choose Destination
Int J Oncol. 2018 Mar 8. doi: 10.3892/ijo.2018.4312. [Epub ahead of print]

FLVCR1 promotes the proliferation and tumorigenicity of synovial sarcoma through inhibiting apoptosis and autophagy.

Author information

1
Department of Orthopaedics, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
2
Department of Nephrology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
3
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, P.R. China.
4
Department of Pathology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
5
Department of Hematology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
6
Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.

Abstract

Feline leukemia virus subgroup C receptor 1 (FLVCR1) has been reported to have a crucial role in variety of biological processes, including cell proliferation, cell death, apoptosis, oxidative stress response, cellular differentiation and metabolism. However, little is known about its role in synovial sarcoma (SS). In the current study, FLVCR1 expression was analyzed in two SS cell lines (SW982 and HS-SY-II), and in eight SS tissues and paired adjacent non-tumor tissues using reverse transcription-quantitative polymerase chain reaction, western blot analysis and immunohistochemistry. Lentivirus-mediated short hairpin RNAs were used to knock down FLVCR1 expression in SW982 and HS-SY-II cells. The effects of FLVCR1 knockdown on the cell proliferation, clonogenicity, cell cycle and apoptosis in SS cells were evaluated by MTT, colony formation assay, flow cytometry analysis, western blotting and in vivo tumorigenesis in nude mice. In the current study, gene expression of FLVCR1 was upregulated in SS cell lines (SW982 and HS-SY-II) and SS tissues from patients. The protein levels of FLVCR1 in SS tissues were also significantly higher than in adjacent non-tumor tissues. Furthermore, suppressing the expression of FLVCR1 in SS cells using short hairpin RNA effectively attenuated cell proliferation, colony formation and impaired the cell cycle, and also significantly induced apoptosis and autophagy. In accordance with this, an in vivo tumorigenicity assay in mice demonstrated that suppression of FLVCR1 expression inhibited the growth of SS tumors implanted subcutaneously. Collectively, these results demonstrated that FLVCR1 may act as an oncoprotein, and have key roles in promoting proliferation and tumorigenicity of SS, and this may shed new light on finding novel therapeutic strategies against SS.

PMID:
29532854
DOI:
10.3892/ijo.2018.4312

Supplemental Content

Full text links

Icon for Spandidos Publications
Loading ...
Support Center