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Nat Cell Biol. 2018 Apr;20(4):432-442. doi: 10.1038/s41556-018-0056-9. Epub 2018 Mar 12.

Daughter-cell-specific modulation of nuclear pore complexes controls cell cycle entry during asymmetric division.

Kumar A1,2, Sharma P1,2, Gomar-Alba M3,4,5,6, Shcheprova Z1,2, Daulny A1,2, Sanmartín T1,2, Matucci I1,2, Funaya C7, Beato M1,2, Mendoza M8,9,10,11,12,13.

Author information

1
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.
2
Universitat Pompeu Fabra (UPF), Barcelona, Spain.
3
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
4
Centre National de la Recherche Scientifique, UMR7104, Illkirch, France.
5
Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France.
6
Université de Strasbourg, Strasbourg, France.
7
European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
8
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain. mendozam@igbmc.fr.
9
Universitat Pompeu Fabra (UPF), Barcelona, Spain. mendozam@igbmc.fr.
10
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France. mendozam@igbmc.fr.
11
Centre National de la Recherche Scientifique, UMR7104, Illkirch, France. mendozam@igbmc.fr.
12
Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France. mendozam@igbmc.fr.
13
Université de Strasbourg, Strasbourg, France. mendozam@igbmc.fr.

Abstract

The acquisition of cellular identity is coupled to changes in the nuclear periphery and nuclear pore complexes (NPCs). Whether and how these changes determine cell fate remain unclear. We have uncovered a mechanism that regulates NPC acetylation to direct cell fate after asymmetric division in budding yeast. The lysine deacetylase Hos3 associates specifically with daughter cell NPCs during mitosis to delay cell cycle entry (Start). Hos3-dependent deacetylation of nuclear basket and central channel nucleoporins establishes daughter-cell-specific nuclear accumulation of the transcriptional repressor Whi5 during anaphase and perinuclear silencing of the G1/S cyclin gene CLN2 in the following G1 phase. Hos3-dependent coordination of both events restrains Start in daughter, but not in mother, cells. We propose that deacetylation modulates transport-dependent and transport-independent functions of NPCs, leading to differential cell cycle progression in mother and daughter cells. Similar mechanisms might regulate NPC functions in specific cell types and/or cell cycle stages in multicellular organisms.

PMID:
29531309
PMCID:
PMC6029668
DOI:
10.1038/s41556-018-0056-9
[Indexed for MEDLINE]
Free PMC Article

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