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Nucleic Acids Res. 2018 May 18;46(9):4382-4391. doi: 10.1093/nar/gky147.

Implications of CpG islands on chromosomal architectures and modes of global gene regulation.

Author information

1
Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas 78712, USA.
2
Kathryn W. Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory, Bar Harbor, Maine 04609, USA.
3
Texas Advanced Computing Center, The University of Texas at Austin, Austin, Texas 78712, USA.
4
Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA.
5
Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, Texas 78712, USA.

Abstract

CpG islands (CGIs) have long been implicated in the regulation of vertebrate gene expression. However, the involvement of CGIs in chromosomal architectures and associated gene expression regulations has not yet been thoroughly explored. By combining large-scale integrative data analyses and experimental validations, we show that CGIs clearly reconcile two competing models explaining nuclear gene localizations. We first identify CGI-containing (CGI+) and CGI-less (CGI-) genes are non-randomly clustered within the genome, which reflects CGI-dependent spatial gene segregation in the nucleus and corresponding gene regulatory modes. Regardless of their transcriptional activities, CGI+ genes are mainly located at the nuclear center and encounter frequent long-range chromosomal interactions. Meanwhile, nuclear peripheral CGI- genes forming heterochromatin are activated and internalized into the nuclear center by local enhancer-promoter interactions. Our findings demonstrate the crucial implications of CGIs on chromosomal architectures and gene positioning, linking the critical importance of CGIs in determining distinct mechanisms of global gene regulation in three-dimensional space in the nucleus.

PMID:
29529258
PMCID:
PMC5961348
DOI:
10.1093/nar/gky147
[Indexed for MEDLINE]
Free PMC Article

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