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Environ Sci Pollut Res Int. 2018 May;25(15):14544-14550. doi: 10.1007/s11356-018-1686-y. Epub 2018 Mar 11.

The toxicity of cadmium ion (Cd2+) to phycocyanin: an in vitro spectroscopic study.

Author information

1
Department of Environmental Engineering, Harbin Institute of Technology, Weihai, 2# Wenhua West Road, Weihai, 264209, People's Republic of China. zhenxingchi@hit.edu.cn.
2
Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, Guangzhou, 510632, People's Republic of China. zhenxingchi@hit.edu.cn.
3
Department of Environmental Engineering, Harbin Institute of Technology, Weihai, 2# Wenhua West Road, Weihai, 264209, People's Republic of China.
4
Department of Civil and Environmental Engineering, University of Technology Sydney, Sydney, 2007, Australia.

Abstract

The pollution of heavy metals is a severer problem for the ecosystems in waters. The toxicity of Cd2+ on phycocyanin (PC) is studied in molecular level in this work. The fluorescence quenching of PC is observed by the adding Cd2+ from 0 to 500 × 10-7 mol L-1. From the theoretical calculation and the time-resolved fluorescence decay profiles, the fluorescence quenching of PC by Cd2+ is found to be static. The synchronous fluorescence spectra are used to study the change in amino acid residues of PC molecules, indicating that the effect of Cd2+ on the Trp of PC is more significant than the Tyr. The UV-Vis absorbance of tetrapyrrole decreases from 0.26 to 0.23 cps with increasing Cd2+ concentration, suggesting that Cd2+ affects the light adsorption and the photosynthesis function of PC. The circular dichroism spectra reveal that adding Cd2+ also changes the secondary structure (α-helix) of PC.

KEYWORDS:

Cd2+; Fluorescence characteristics; Molecule structure; Phycocyanin; Toxicity

PMID:
29527648
DOI:
10.1007/s11356-018-1686-y
[Indexed for MEDLINE]

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