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Bioresour Technol. 2018 Jun;258:234-239. doi: 10.1016/j.biortech.2018.03.006. Epub 2018 Mar 2.

Optimized co-production of lipids and carotenoids from Ettlia sp. by regulating stress conditions.

Author information

1
Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Environmental Biotechnology, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
2
Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
3
Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Environmental Biotechnology, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. Electronic address: heemock@kribb.re.kr.

Abstract

This study used a single strain Ettlia sp. YC001 and two stages to optimize the production of three materials: lipids, lutein, and β-carotene. In the cultivation stage for lutein production, different temperatures, light qualities, and intensities were applied. The highest biomass was obtained at 35 °C, but the maximum lutein productivity of 6.1 mg/L/d achieved at 25 °C. In the stress stage for lipids and β-carotene production, UV-A and nitrogen starvation were applied. While UV stress increased the chlorophyll-a and β-carotene content. The β-carotene, oleic acid, and lipids significantly increased under nitrogen starvation with a high light intensity of 1200 µmol/m2/s, plus the Ettlia sp. changed from green to red. The results showed that Ettlia sp. can be an effective microalga for the co-production of lutein, β-carotene, and biodiesel.

KEYWORDS:

Carotenoids; Ettlia; Light stress; Lipid; Temperature

PMID:
29525599
DOI:
10.1016/j.biortech.2018.03.006
[Indexed for MEDLINE]

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