Format

Send to

Choose Destination
Methods Mol Biol. 2018;1767:271-288. doi: 10.1007/978-1-4939-7774-1_15.

Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis.

Author information

1
Department of Surgery and Cancer, Imperial College London, London, UK.
2
Department of Bariatric and Emergency General Surgery, Homerton University Hospital, London, UK.
3
Department of Surgery and Cancer, Imperial College London, London, UK. l.magnani@imperial.ac.uk.

Abstract

Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become an essential tool for epigenetic scientists. ChIP-seq is used to map protein-DNA interactions and epigenetic marks such as histone modifications at the genome-wide level. Here we describe a complete ChIP-seq laboratory protocol (tailored toward processing tissue samples as well as cell lines) and the bioinformatic pipelines utilized for handling raw sequencing files through to peak calling.

KEYWORDS:

Antibodies; Bioinformatic pipelines; Bioinformatics; ChIP-seq; ChIP-seq data processing; Chromatin immunoprecipitation and high-throughput sequencing; DNA library assembly; Genome alignment; Peak calling

PMID:
29524141
DOI:
10.1007/978-1-4939-7774-1_15
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center