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J Chromatogr Sci. 2018 Mar 7. doi: 10.1093/chromsci/bmy016. [Epub ahead of print]

Performance of Optimized Wide Pore Superficially Porous Particles for Separation of Proteins and Immunoglobulin G Antibodies.

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1
Agilent Technologies, Inc., 2850 Centerville Road, Wilmington, DE 19808, USA.

Abstract

In this study, we studied the chromatographic performance of this newly developed wide pore superficially porous particles (SPPs) with 3.5 μm particle size and 450 Å pore size, for the separation of proteins and Immunoglobulin G antibodies. We studied the selectivity of different phases (C4, SB-C18 and Diphenyl), the effect of temperature, column carryover and column chemical lifetime. We also compared our SPPs with other wide pore SPPs in similar particle sizes and sub 2 µ wide pore totally porous particles by van Deemter studies and gradient separations of proteins and immunoglobulin G antibodies. The results showed that the SPPs containing larger pore size gave better chromatographic performance.

PMID:
29522182
DOI:
10.1093/chromsci/bmy016

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