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Hematology. 2018 Oct;23(9):705-711. doi: 10.1080/10245332.2018.1446703. Epub 2018 Mar 9.

Causes of microcytic anaemia and evaluation of conventional laboratory parameters in the differentiation of erythrocytic microcytosis in blood donors candidates.

Author information

1
a Department of Medicine , Universidade Federal do Triângulo Mineiro (UFTM) , Uberaba , Minas Gerais , Brazil.
2
b Department of Nursing , Universidade Federal do Triângulo Mineiro (UFTM) , Uberaba , Minas Gerais , Brazil.
3
c Department of Medicine , Universidade Federal do Triângulo Mineiro (UFTM) , Uberaba , Minas Gerais , Brazil.

Abstract

CONTEXT AND OBJECTIVE:

Microcytic anaemia results from defective synthesis of haemoglobin in the erythroid precursors, causing a reduction in its mean corpuscular volume (MCV). The most common causes of microcytosis, without the increase in HbA2 levels, are iron deficiency anaemia (IDA) and α-thalassemia. The aim of this study was to identify the causes of microcytic anaemia and evaluate the haematological parameters from blood donors deemed ineligible (due to the low haematocrit level) that would differentiate the IDA and α-thal, whether isolated or in association.

METHODS:

Genomic DNA was submitted to the polymerase chain reaction multiplex for the diagnosis of the most common allele deletions of α-thal and erythrogram and in order to verify haematological parameters. Iron deficiency (ID) was determined through the measurement of serum ferritin.

RESULTS:

Of the 204 samples, 82 (40.2%) were identified with ID, 24 (17.8%) with α-thal and 10 (4.9%) with ID associated with α-thal. In the α-thal with ID group haemoglobin (Hb), MCV, mean corpuscular Hb concentration (MCHC) and mean corpuscular Hb (MCH) values were significantly lower compared to the isolated α-thal. In the group with ID Hb, MCV, MCHC and MCH values were significantly lower compared to those with isolated α-thal. The α-thal with ID group, showed Hb, MCV, MCHC and MCH significantly reduced when compared to those with IDA.

CONCLUSIONS:

This study showed that the values of haematological parameters, especially haematocrit, Hb, MCV, MCH, MCHC and red blood cell distribution width (RDW), are lower in patients with IDA, especially when associated with α-thal and therefore it may be useful to discriminate between the different types of microcytic anaemia.

KEYWORDS:

Anaemia; anaemia; diagnosis; differential; erythrocyte indices; hypochromic microcytic; iron deficiency; thalassemia

PMID:
29521164
DOI:
10.1080/10245332.2018.1446703
[Indexed for MEDLINE]

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