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Pharm Biol. 2018 Dec;56(1):176-182. doi: 10.1080/13880209.2018.1446029.

Protective properties of geniposide against UV-B-induced photooxidative stress in human dermal fibroblasts.

Author information

1
a R&D Center, Cosmocos Corporation , Incheon , Republic of Korea.
2
b College of Pharmacy and Research Institute of Pharmaceutical Sciences , Seoul National University , Seoul , Republic of Korea.
3
c Department of Biochemistry , Kangwon National University , Chuncheon , Republic of Korea.
4
d R&D Center, Shebah Biotech Inc , Chuncheon , Republic of Korea.
5
e KISSMYSKIN Co , Busan , Republic of Korea.

Abstract

CONTEXT:

Geniposide (genipin-1-O-β-d-glucoside) is a major bioactive ingredient in the fruits of gardenia [Gardenia jasminoides J. Ellis (Rubiaceae)], a traditional herbal medicine in Asian countries.

OBJECTIVE:

This work assesses the skin anti-photoaging potential of geniposide in human dermal fibroblasts under UV-B irradiation.

MATERIALS AND METHODS:

The anti-photoaging property of geniposide, at varying concentrations (5, 12 and 30 μM) treated for 30 min prior to UV-B irradiation, was evaluated by analysing reactive oxygen species (ROS), promatrix metalloproteinase-2 (proMMP-2), glutathione (GSH), superoxide dismutase (SOD), nuclear factor erythroid 2-related factor 2 (Nrf2) and cellular viability.

RESULTS:

Geniposide suppressed the ROS elevation under UV-B irradiation, which was revealed using three ROS-sensitive fluorescent dyes. The use of 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA), dihydroethidium (DHE) and dihydrorhodamine 123 (DHR-123) elicited the IC50 values of 10.5, 9.8 and 21.0 μM, respectively. Geniposide attenuated proMMP-2 at activity and protein levels that were elevated under UV-B-irradiation. Geniposide at 5, 12 and 30 μM augmented the UV-B-reduced total GSH content to 1.9 ± 0.1-, 2.2 ± 0.2- and 4.1 ± 0.2-fold, respectively. Geniposide at 5, 12 and 30 μM upregulated total SOD activity to 2.3 ± 0.1-, 2.5 ± 0.3- and 3.3 ± 0.3-fold, respectively, under UV-B irradiation. The UV-B-reduced Nrf2 levels were also upregulated by geniposide treatment. Geniposide, at the concentrations used, was unable to interfere with cellular viabilities under UV-B irradiation.

DISCUSSION AND CONCLUSIONS:

After the skin anti-photoaging potential of geniposide may be further verified, it can be utilized as a safer resource in the manufacture of effective anti-aging cosmetics.

KEYWORDS:

Anti-photoaging; Nrf2; glutathione; matrix metalloproteinase-2; reactive oxygen species; superoxide dismutase

PMID:
29521149
PMCID:
PMC6130509
DOI:
10.1080/13880209.2018.1446029
[Indexed for MEDLINE]
Free PMC Article

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