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Eur J Immunol. 2018 Jun;48(6):937-949. doi: 10.1002/eji.201747162. Epub 2018 Apr 30.

Miscarriage induced by adoptive transfer of dendritic cells and invariant natural killer T cells into mice.

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Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan.
Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan.


Unexpected fetal loss is one of the common complications of pregnancy; however, the pathogenesis of many miscarriages, particularly those not associated with infections, is unknown. We previously found that activated DEC-205+ dendritic cells (DCs) and NK1.1+ invariant natural killer T (iNKT) cells are recruited into the myometrium of mice when miscarriage is induced by the intraperitoneal administration of α-galactosylceramide (α-GalCer). Here we demonstrate that the adoptive transfer of DEC-205+ bone marrow-derived DCs cocultured with α-GalCer (DEC-205+ BMDCs-c/w-α-GalCer) directly induced marked fetal loss by syngeneic pregnant C57BL/6 (B6) mice and allogeneic mice (B6 (♀) × BALB/c (♂)), which was accompanied by the accumulation of activated iNKT cells in the myometrium. Further, the adoptive transfer of NK1.1+ iNKT cells obtained from B6 mice injected with α-GalCer facilitated miscarriages in syngeneic Jα18(-/-) (iNKT cell-deficient) mice. These results suggest that DEC-205+ DCs and NK1.1+ iNKT cells play crucial roles required for the initiation of fetal loss associated with stimulation by glycolipid antigens and sterile inflammation.


Adoptive transfer; Dendritic cell (DC); Invariant NKT cell (iNKT cell); Miscarriage; α-galactosylceramide (α-GalCer)

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