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JACC Basic Transl Sci. 2017 Oct;2(5):543-560. doi: 10.1016/j.jacbts.2017.03.016. Epub 2017 Oct 30.

Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status.

Author information

1
Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland.
2
Division of Cardiology, University of California San Francisco, San Francisco, California.
3
Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee.
4
Department of Radiology, Washington University School of Medicine, St. Louis, Missouri.
5
Department of Radiology, Johns Hopkins School of Medicine, Baltimore, Maryland.
6
National Institute on Aging/National Institutes of Health, Baltimore, Maryland.

Abstract

Adult stem cells demonstrate metabolic flexibility that is regulated by cell adhesion status. The authors demonstrate that adherent cells primarily utilize glycolysis, whereas suspended cells rely on oxidative phosphorylation for their ATP needs. Akt phosphorylation transduces adhesion-mediated regulation of energy metabolism, by regulating translocation of glucose transporters (GLUT1) to the cell membrane and thus, cellular glucose uptake and glycolysis. Cell dissociation, a pre-requisite for cell transplantation, leads to energetic stress, which is mediated by Akt dephosphorylation, downregulation of glucose uptake, and glycolysis. They designed hydrogels that promote rapid cell adhesion of encapsulated cells, Akt phosphorylation, restore glycolysis, and cellular ATP levels.

KEYWORDS:

SPECT imaging; cardiosphere-derived cells (CDCs); glycolysis; metabolism; oxidative phosphorylation (OxPhos); sodium-iodide symporter (NIS)

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