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Nat Commun. 2018 Mar 8;9(1):994. doi: 10.1038/s41467-018-03408-4.

Expansion of functional personalized cells with specific transgene combinations.

Author information

1
Model Systems for Infection and Immunity, HZI - Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.
2
Experimental Cardiology, Justus-Liebig University Giessen, Aulweg 129, 35392, Giessen, Germany.
3
Department of Gene Regulation and Differentiation, HZI - Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.
4
InSCREENeX GmbH, Inhoffenstr. 7, 38124, Braunschweig, Germany.
5
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7, 38124, Braunschweig, Germany.
6
Experimental Immunology, HZI - Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.
7
Department of Gastroenterology, Hepatology, Endocrinology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
8
Translational Research Group Cell and Gene Therapy, Twincore - Centre for Experimental and Clinical Infection Research GmbH, Feodor-Lynen-Str. 7, 30625, Hannover, Germany.
9
Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, MHH, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
10
Research Core Unit Genomics, Medical School Hannover, 30625, Hannover, Germany.
11
Department of Anatomy and Cell Biology, RWTH Aachen University, 52074, Aachen, Germany.
12
Department of Orthopaedics, Aachen University Hospital, RWTH Aachen University, Aachen, 52074, Germany.
13
Department of Orthopedic Surgery of the Lower Limb and Arthroplasty, Rummelsberg Hospital, Schwarzenbruck, 90592, Germany.
14
Department of Internal Medicine, Hospital U.M. Valdecilla, University of Cantabria, IDIVAL, 39008, Santander, Spain.
15
Institute of Physiology and Pathophysiology, RG Blood Vessel Remodeling, University Heidelberg, Im Neuenheimer Feld 326, 69120, Heidelberg, Germany.
16
Department of Hematology, West German Cancer Center (WTZ), University Hospital Essen, Hufelandstr. 55, 45147, Essen, Germany.
17
Institute for Transfusion Medicine, University Hospital Essen, Virchowstr. 179, 45147, Essen, Germany.
18
Instituto de Biologia Experimental e Tecnologica, Universidade Nova de Lisboa, Oeiras, 2781-901, Portugal.
19
Model Systems for Infection and Immunity, HZI - Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany. dagmar.wirth@helmholtz-hzi.de.
20
Experimental Hematology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany. dagmar.wirth@helmholtz-hzi.de.
21
InSCREENeX GmbH, Inhoffenstr. 7, 38124, Braunschweig, Germany. tobias.may@inscreenex.com.

Abstract

Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.

PMID:
29520052
PMCID:
PMC5843645
DOI:
10.1038/s41467-018-03408-4
[Indexed for MEDLINE]
Free PMC Article

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