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Mater Sci Eng C Mater Biol Appl. 2018 Mar 1;84:12-20. doi: 10.1016/j.msec.2017.11.002. Epub 2017 Nov 11.

Organic composite-mediated surface coating of human acellular bone matrix with strontium.

Author information

1
Laboratory of Stem Cell and Tissue Engineering, State Key laboratory of biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 60041, China.
2
School of Materials Science and Engineering, Tianjin University, Tianjin 300350, China.
3
Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies, Orthopaedic Research Center, Peking University Shenzhen Hospital, Shenzhen 518036, China; Shenzhen Key Laboratory of Spine Surgery, Department of Spine Surgery, Peking University Shenzhen Hospital, Shenzhen 518036, China; Department of Orthopaedics and Traumatology, The University of Hong Kong, Hong Kong SAR 999077, China.
4
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 60041, China.
5
Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
6
School of Materials Science and Engineering, Tianjin University, Tianjin 300350, China. Electronic address: zyli@tju.edu.cn.
7
Laboratory of Stem Cell and Tissue Engineering, State Key laboratory of biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 60041, China. Electronic address: dengli2000@gmail.com.

Abstract

Acellular bone matrix (ACBM) provides an osteoconductive scaffold for bone repair, but its osteoinductivity is poor. Strontium (Sr) improves the osteoinductivity of bone implants. In this study, we developed an organic composite-mediated strontium coating strategy for ACBM scaffolds by using the ion chelating ability of carboxymethyl cellulose (CMC) and the surface adhesion ability of dopamine (DOPA). The organic coating composite, termed the CMC-DOPA-Sr composite, was synthesized under a mild condition, and its chemical structure and strontium ion chelating ability were then determined. After surface decoration, the physicochemical properties of the strontium-coated ACBM (ACBM-Sr) scaffolds were characterized, and their biocompatibility and osteoinductivity were determined in vitro and in vivo. The results showed that the CMC-DOPA-Sr composite facilitated strontium coating on the surface of ACBM scaffolds. The ACBM-Sr scaffolds possessed a sustained strontium ion release profile, exhibited good cytocompatibility, and enhanced the osteogenic differentiation of mesenchymal stem cells in vitro. Furthermore, the ACBM-Sr scaffolds showed good histocompatibility after subcutaneous implantation in nude mice. Taken together, this study provided a simple and mild strategy to realize strontium coating for ACBM scaffolds, which resulted in good biocompatibility and improved osteoinductivity.

KEYWORDS:

Acellular bone matrix; Biocompatibility; Osteoinductivity; Strontium; Surface coating

PMID:
29519420
DOI:
10.1016/j.msec.2017.11.002
[Indexed for MEDLINE]

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