Effect of golden needle mushroom (Flammulina velutipes) stem waste on laying performance, calcium utilization, immune response and serum immunity at early phase of production

Shad Mahfuz; Hui Song; Zhongjun Liu; Xinyu Liu; Zipeng Diao; Guihong Ren; Zhixin Guo; Yan Cui

doi:10.5713/ajas.17.0787

Effect of golden needle mushroom (Flammulina velutipes) stem waste on laying performance, calcium utilization, immune response and serum immunity at early phase of production

Asian-Australas J Anim Sci. 2018 May;31(5):705-711. doi: 10.5713/ajas.17.0787. Epub 2018 Mar 2.

Authors

Shad Mahfuz^{1

2}, Hui Song^{1

3}, Zhongjun Liu⁴, Xinyu Liu¹, Zipeng Diao¹, Guihong Ren^{1

5}, Zhixin Guo^{1

6}, Yan Cui^{1

7}

Affiliations

¹ School of Life Science, Jilin Agricultural University, Changchun 130118, China.
² Department of Animal Nutrition, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
³ Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Changchun 130118, China.
⁴ College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China.
⁵ School of Chemistry and Biology, Beihua University, Jilin 132013, China.
⁶ College of Live Science, Tonghua Normal University, Tonghua, Jilin 134001, China.
⁷ College of Animal Science and Veterinary Medicine, Jinzhou Medical University, Jinzhou 121001, China.

Abstract

Objective: This experiment was conducted to evaluate the effects of golden needle mushroom (Flammulina velutipes) stem waste (FVW), on organic eggs production, calcium utilization, antibody response, serum immunoglobulin, and serum cytokine concentration at early phase of production in laying hens.

Methods: A total 210, 19 weeks old aged ISA Brown layers were randomly assigned into 5 equal treatment groups, with 7 replications of 6 hens each. Dietary treatment included a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4% FVW; and 6% FVW. The experimental duration was 10 weeks.

Results: There was no significant differences (p>0.05) on hen day egg production, egg weight, egg mass, feed intake, and feed conversion ratio (FCR) among experimental groups. Unmarketable eggs were significantly lower (p<0.05) both in 4% FVW and 6% FVW fed groups than control group. The calcium retention and calcium in egg shell deposition were significantly higher (p<0.05) in FVW inclusion groups than control and antibiotic groups. Antibody titers against Newcastle diseases were significantly higher (p<0.05) in 6% FVW fed group (except combined with 4% FVW at day 147) and infectious bronchitis were significantly higher (p< 0.05) in FVW fed groups (except 2% FVW and 4% FVW at day 161) than control and antibiotic groups. Serum immunoglobulin sIgA was significantly higher (p<0.05) in all levels of FVW and IgG was significantly higher (p<0.05) in 4% FVW than control and antibiotic groups. Serum cytokine concentration interleukin-2 (IL-2) was significantly higher (p<0.05) in 6% FVW; IL-6 and tumor necrotic factor-α were significantly higher (p<0.05) both in 4% FVW and 6% FVW than control and antibiotic groups; IL-4 was significantly higher (p<0.05) in antibiotic, 2% FVW and 4% FVW fed groups than control.

Conclusion: F. velutipes mushroom waste can be used as a novel substitute for antibiotic for organic egg production and sound health status in laying hens.

Keywords: Antibody Response; Calcium Utilization; Laying Hens; Laying Performance; Mushroom Waste; Serum Immunity.