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Elife. 2018 Mar 7;7. pii: e32723. doi: 10.7554/eLife.32723.

TANGO1 builds a machine for collagen export by recruiting and spatially organizing COPII, tethers and membranes.

Author information

1
Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain.
2
Universitat Pompeu Fabra, Barcelona, Spain.
3
SIMBIOsys Group, Department of Information and Communication Technologies, Universitat Pompeu Fabra, Barcelona, Spain.
4
ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Castelldefels, Spain.
5
Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain.
#
Contributed equally

Abstract

Collagen export from the endoplasmic reticulum (ER) requires TANGO1, COPII coats, and retrograde fusion of ERGIC membranes. How do these components come together to produce a transport carrier commensurate with the bulky cargo collagen? TANGO1 is known to form a ring that corrals COPII coats, and we show here how this ring or fence is assembled. Our data reveal that a TANGO1 ring is organized by its radial interaction with COPII, and lateral interactions with cTAGE5, TANGO1-short or itself. Of particular interest is the finding that TANGO1 recruits ERGIC membranes for collagen export via the NRZ (NBAS/RINT1/ZW10) tether complex. Therefore, TANGO1 couples retrograde membrane flow to anterograde cargo transport. Without the NRZ complex, the TANGO1 ring does not assemble, suggesting its role in nucleating or stabilising this process. Thus, coordinated capture of COPII coats, cTAGE5, TANGO1-short, and tethers by TANGO1 assembles a collagen export machine at the ER.

KEYWORDS:

Multisubunit Tether Complex; STED; cell biology; extracellular matrix; human; lineactant; molecular biophysics; secretion; structural biology

PMID:
29513218
PMCID:
PMC5851698
DOI:
10.7554/eLife.32723
[Indexed for MEDLINE]
Free PMC Article

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