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Am J Transl Res. 2018 Feb 15;10(2):592-604. eCollection 2018.

Extensive profiling of circular RNAs and the potential regulatory role of circRNA-000284 in cell proliferation and invasion of cervical cancer via sponging miR-506.

Author information

1
Department of Obstetrics and Gynecology, Tongji Hospital, School of Medicine, Tongji University389 Xin Cun Road, Shanghai 200065, China.
2
Stem Cell Translational Research Center, Tongji Hospital, School of Medicine, Tongji UniversityShanghai 200065, China.
3
College of Life Sciences, Nanchang UniversityNanchang 330031, China.
4
Department of Obstetrics and Gynecology, Liyang People's HospitalChangzhou 213300, Jiangsu, China.

Abstract

Circular RNAs (circRNAs) are key regulators in the development and progression of human cancers, however its role in cervical cancer tumorigenesis is not well understood. The present study aims to investigate the expression profiles and potential modulation of circRNA on cervical cancer carcinogenesis. Human circRNA microarray was performed to screen for abnormally expressed circRNA in cervical cancer cells and circRNA-000284 was identified as one circRNA significantly upregulated in cervical cancer cells. Subsequent mechanistic investigations suggested that knockdown of circRNA-000284 suppressed cell proliferation and invasion, and caused G0/G1 phase cell cycle arrest. By performing anti-AGO2 RNA precipitation and luciferase reporter assay, we identified miR-506 as the circRNA-000284-associated miRNA. Furthermore, Snail-2 was identified as a direct target of miR-506, and circRNA-000284 could positively regulate the expression of Snail-2. Finally, the tumor promoting effect of circRNA-000284 was abolished by co-expression of miR-506 mimics or Snail-2 silencing vector. In conclusion, circRNA-000284 promotes cell proliferation and invasion in cervical cancer, and may serve as a promising therapeutic target for cervical cancer patients. Therefore, silence of circRNA-000284 could be a future direction to develop a novel treatment strategy.

KEYWORDS:

Cervical cancer; Snail-2; circRNA-000284; miR-506

PMID:
29511454
PMCID:
PMC5835825

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