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BMC Infect Dis. 2018 Mar 6;18(1):113. doi: 10.1186/s12879-018-3015-y.

Next generation sequencing based pathogen analysis in a patient with neurocysticercosis: a case report.

Author information

1
Department of Neurology, First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China.
2
BGI-Shenzhen, Main Building, Bei Shan Industrial Zone, Yantian District, Shenzhen, Guangdong, 518083, China.
3
BGI-Shanghai, 8th Floor, 26th Building, 3399 Lane, Kangxin Road, Pudong District, Shanghai, 200120, China.
4
Binhai Genomics Institute, Tianjin Translational Genomics Center, BGI-Tianjin, Tianjin, 300308, China.
5
Department of Neurology, First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China. guopingpeng@zju.edu.cn.

Abstract

BACKGROUND:

Accurate and early diagnosis of neurocysticercosis (NCC) remains a challenge due to the heterogeneity of its clinical, immunological and imaging characteristics. The presence of cysticercus DNA in cerebrospinal fluid (CSF) of NCC patients has been previously detected via conventional PCR assays. To the best of our knowledge, the use of CSF Next-Generation Sequencing (NGS) based pathogen analysis in patients with NCC infection has never been reported.

CASE PRESENTATION:

This study reports the clinical, imaging, and immunological features of a patient initially presenting with several months of headache who further developed a pure sensory stroke. NGS was used to detect the pathogen, and her CSF demonstrated the presence of Taenia solium-DNA. This finding was confirmed by a positive reaction to CSF cysticercosis antibodies. After antiparasitic treatment, secondary CSF NGS revealed the DNA index have dropped considerably compared to the initial NGS readings.

CONCLUSIONS:

NGS is a promising tool for the early and accurate diagnosis of central nervous system (CNS) infection, especially in the setting of atypical clinical manifestations. Further studies are required to evaluate the persistence of DNA in the CSF of patients.

KEYWORDS:

Headache; Neurocysticercosis; Next generation sequencing; Sensory stroke; Taenia solium

PMID:
29510659
PMCID:
PMC5840791
DOI:
10.1186/s12879-018-3015-y
[Indexed for MEDLINE]
Free PMC Article

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