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Mar Drugs. 2018 Mar 6;16(3). pii: E82. doi: 10.3390/md16030082.

Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway.

Liu Y1,2,3, Geng L4,5,6, Zhang J7, Wang J8,9, Zhang Q10,11,12, Duan D13,14, Zhang Q15,16.

Author information

1
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. liuyingjuan829@163.com.
2
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. liuyingjuan829@163.com.
3
University of the Chinese Academy of Sciences, Beijing 100049, China. liuyingjuan829@163.com.
4
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. genglihua13@mails.ucas.ac.cn.
5
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. genglihua13@mails.ucas.ac.cn.
6
University of the Chinese Academy of Sciences, Beijing 100049, China. genglihua13@mails.ucas.ac.cn.
7
Pharmaceutical Department, Qingdao Eighth People's Hospital, Qingdao 266000, China. zhangjingjing02@126.com.
8
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. jingwang@qdio.ac.cn.
9
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. jingwang@qdio.ac.cn.
10
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. zhangqi515@mails.ucas.ac.cn.
11
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. zhangqi515@mails.ucas.ac.cn.
12
University of the Chinese Academy of Sciences, Beijing 100049, China. zhangqi515@mails.ucas.ac.cn.
13
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. dlduan@qdio.ac.cn.
14
State Key Lab of Seaweed Bioactive Substances, Qingdao 266000, China. dlduan@qdio.ac.cn.
15
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. qbzhang@qdio.ac.cn.
16
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. qbzhang@qdio.ac.cn.

Abstract

Parkinson's disease (PD) is a neurodegenerative movement disorder that is caused by a selective loss of dopaminergic neurons. Current PD treatments provide symptomatic relief but do not prevent or decelerate disease progression. Previous studies have suggested that acetylated and phosphorylated porphyran, derived from Porphyra, produces a neuroprotective effect against 6-OHDA-induced damage. Due to its antioxidant and neuroprotective potential, this study evaluates whether oligo-porphyran (OP) could be beneficial in an experimental model of PD in mice. The drug 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was intraperitoneally injected (20 mg/kg body weight) for seven days to simulate PD, followed by OP administration. We found that the behavioral deficits in spontaneous motor activity, latency to descend in a pole test, and suspension in a traction test were ameliorated, and excessive dopamine (DA) metabolism was suppressed after OP treatment. Additionally, we found that OP protected dopaminergic neurons by preventing MPTP-induced decreases in dopaminergic transporter and tyrosine hydroxylase protein levels. We speculated whether OP regulates a signaling pathway that affects the behavioral changes seen in PD mice. In this study, the PI3K/Akt/Bcl-2 pathway was detected. Our results demonstrate that OP increased the phosphorylation of PI3K/Akt/GSK-3β and inhibited the activation of caspase-3 and poly (ADP-ribose) polymerase, with changes in the Bax/Bcl-2 ratio. These results showed that OP might promote DA neuron survival in vivo by regulating the PI3K/Akt/Bcl-2 pathway, thereby ameliorating the neurobehavioral deficits in a PD mouse model and suggesting OP as a neuroprotective treatment for PD.

KEYWORDS:

PI3K/Akt; Parkinson’s disease; apoptosis; neurobehavior; oligo-porphyran

PMID:
29509717
PMCID:
PMC5867626
DOI:
10.3390/md16030082
[Indexed for MEDLINE]
Free PMC Article

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