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Proteomics Clin Appl. 2018 Sep;12(5):e1700012. doi: 10.1002/prca.201700012. Epub 2018 Apr 3.

Effects of let-7e on LPS-Stimulated THP-1 Cells Assessed by iTRAQ Proteomic Analysis.

Gui L1,2,3, Zhang Q1,2,3, Cai Y1,2, Deng X1,2, Zhang Y1,2,3, Li C1,2, Guo Q1,2, He X1,2, Huang J1,2,3.

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Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P. R. China.
Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P. R. China.
Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P. R. China.



Previous studies have demonstrated that let-7e is associated with inflammatory responses. To date, the roles and mechanisms of let-7e have not been completely revealed.Therefore, we aim to identify proteins associated with let-7e overexpression and explore their functions in the immune responses, including in cytokine production.


High-throughput isobaric tag for relative and absolute quantitation (iTRAQ) technology is used to provide the first genome-wide study of THP-1 cells transfected with let-7e mimic followed by lipopolysaccharide (LPS) stimulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database and KEGG pathway enrichment analyses are used to analyze a large number of differentially expressed proteins (DEPs) associated with let-7e overexpression or LPS stimulation. Quantitative reverse transcription PCR (qRT-PCR) and 50% tissue culture infective dose (TCID50) assays are processed to confirm the relationship of let-7e and dengue virus replication.


iTRAQ results show that let-7e is associated with the expression of anti-viral proteins. What's more, calcineurin subunit B type 1, an anti-tumor factor, is upregulated by let-7e after LPS stimulation. KEGG analyses identify that some DEPS associated with let-7e overexpression are involved in the measles and influenza A pathways, and LPS-stimulated proteins in THP-1 cells are mainly enriched in transcriptional misregulation in cancer pathway and hippo signaling pathway (multiple species). The results of qRT-PCRand TCID50 show that let-7e promotes dengue virus replication, which is in agreement with the iTRAQ results.


These results provide molecular insights into the regulatory mechanisms of let-7e in cytokine expression, virus replication, and anti-tumor function.


Cytokines; Proteomic; Virus; let-7e

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