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Bull Exp Biol Med. 2018 Mar;164(4):536-542. doi: 10.1007/s10517-018-4028-7. Epub 2018 Mar 4.

Isolation and Characterization of Human Myoblast Culture In Vitro for Technologies of Cell and Gene Therapy of Skeletal Muscle Pathologies.

Author information

1
Research Centre for Medical Genetics, Moscow, Russia. vyutab@yandex.ru.
2
Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
3
Research Centre for Medical Genetics, Moscow, Russia.

Abstract

We analyzed cultures of 5 independent myoblast lines from human skeletal muscles. It was shown that the content of desmin-positive cells in cultures at early passages exceeds 90%. Typical morphofunctional signs of myogenic differentiation disturbances were identified and their dynamics was studied. Signs of alternative adipogenic and chondrogenic differentiation of cells were revealed. Based on these data, limitations for the use of myoblast cultures of certain passages for biomedical research and cell therapy were evaluated.

KEYWORDS:

cultivation; differentiation; myoblasts; satellite cells

PMID:
29504093
DOI:
10.1007/s10517-018-4028-7
[Indexed for MEDLINE]

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