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Food Chem Toxicol. 2018 May;115:20-25. doi: 10.1016/j.fct.2018.02.060. Epub 2018 Feb 28.

Effects of perinatal exposure to bisphenol A on the intraprostatic levels of aromatase and 5α-reductase isozymes in juvenile rats.

Author information

1
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, 11 Avenue of Research, 18016, Granada, Spain.
2
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, 11 Avenue of Research, 18016, Granada, Spain; Faculty of Medicine, Institute of Neurosciences, University of Granada, 11 Avenue of Research, 18016, Granada, Spain. Electronic address: torrespi@ugr.es.
3
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, 11 Avenue of Research, 18016, Granada, Spain; Faculty of Medicine, Institute of Neurosciences, University of Granada, 11 Avenue of Research, 18016, Granada, Spain. Electronic address: esortega@ugr.es.

Abstract

The impact of bisphenol A (BPA) on the prostate gland has taken center stage, with a special focus placed on understanding how BPA affects prostate physiopathology. In this study, we evaluated the ability of lower doses of BPA to induce alterations in 5α-R isozymes and aromatase, in the prostate of juvenile rats exposed during developmental stage. Gestating Wistar rats were treated s.c with either vehicle or BPA (2.4 and 10 μg/kg b.w./day) from gestational day 12 to parturition. Then, male pups were s.c treated from postnatal day 1 through day 21, when they were euthanized and qRT-PCR, western blot and hormone levels determination were performed. We found that BPA at dose of 2.4 and 10 μg/kg b.w./day significantly decreased the mRNA and protein levels of 5α-R2. However, neither 5α-R1 nor 5α-R3 was affected by this exposure. BPA at dose of 10 μg/kg b.w./day significantly increased the mRNA and protein levels of aromatase. BPA also decreased plasma levels of both testosterone and dihydrotestosterone and increased estradiol. These data lend support that low-dose BPA during fetal and neonatal prostate development interfere with in situ estrogen and androgen production in the prostate gland of juvenile rats through the enzymes aromatase and 5α-Reductase.

KEYWORDS:

5α-reductase isozymes; Aromatase; Bisphenol A; Prostate

PMID:
29501275
DOI:
10.1016/j.fct.2018.02.060
[Indexed for MEDLINE]

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