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EBioMedicine. 2018 Mar;29:159-165. doi: 10.1016/j.ebiom.2018.02.020. Epub 2018 Feb 23.

Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment.

Author information

1
MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK.
2
Moredun Research Institute and Napier University, Edinburgh, Midlothian, UK.
3
Moredun Research Institute and the Roslin Institute at the University of Edinburgh, Midlothian, UK.
4
Jefferiss Research Trust Laboratories, Imperial College London, London, UK.
5
Department of Medical Microbiology, North Bristol NHS Trust, Bristol, UK.
6
Department of Obstetrics and Gynecology, The University of Hong Kong, Hong Kong, China.
7
MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK. Electronic address: andrew.horne@ed.ac.uk.

Abstract

Tubal ectopic pregnancies are a leading cause of global maternal morbidity and mortality. Previous infection with Chlamydia trachomatis is a major risk factor for tubal embryo implantation but the biological mechanism behind this association is unclear. Successful intra-uterine embryo implantation is associated with increased expression of endometrial "receptivity" integrins (cell adhesion molecules). We examined integrin expression in Fallopian tubes of women with previous C. trachomatis infection, in mice experimentally infected with C. trachomatis, in immortalised human oviductal epithelial cells (OE-E6/E7) and in an in vitro model of human embryo attachment (trophoblast spheroid-OE-E6/7 cell co-culture). Previous exposure with C. trachomatis increased Fallopian tube/oviduct integrin-subunit beta-1 (ITGB1) in women and mice compared to controls. C. trachomatis increased OE-E6/E7 cell ITGB1 expression and promoted trophoblast attachment to OE-E6/E7 cells which was negated by anti-ITGB1-antibody. We demonstrate that infection with C. trachomatis increases tubal ITGB1 expression, predisposing to tubal embryo attachment and ectopic pregnancy.

KEYWORDS:

Chlamydia trachomatis; Ectopic pregnancy; Embryo implantation; Fallopian tube; Integrins

PMID:
29500127
PMCID:
PMC5925620
DOI:
10.1016/j.ebiom.2018.02.020
[Indexed for MEDLINE]
Free PMC Article

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