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Biosens Bioelectron. 2018 Jun 15;108:53-61. doi: 10.1016/j.bios.2018.02.044. Epub 2018 Feb 21.

Electron beam-irradiated polypyrrole decorated with Bovine serum albumin pores: Simultaneous determination of epinephrine and L-tyrosine.

Author information

1
Polymer Electronics Lab, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630004, Tamilnadu, India.
2
Polymer Electronics Lab, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630004, Tamilnadu, India. Electronic address: wilson.j2008@yahoo.com.

Abstract

In current work highly sensitive and stable electrochemical sensor for simultaneous non-enzymatic detection of epinephrine (EP), L-tyrosine (L-Tyr) is constructed based on Electron beam irradiated Polypyrrole (EB-Ppy) nanospheres (Zeta potential 33.69 mV at pH 7) embedded over bovine serum albumin (BSA) (Zeta potential - 11.54 mV at pH 7) porous structure, fabricated by simple chemical routes. The BSA structure has the advantages of large surface area, excellent structure stability, rich pore channels and redox mediator role. The constructed sensor exhibited excellent sensor performances by the combination of protein with NH group and recorded the linear response of EP, L-Tyr individual in the concentration range of 100 nM-1 mM, 100 nM-800 μM, with detection limit 7.1 nM, 8.8 nM (S/N = 3σ/b). The EB-Ppy-BSA/GCE electrochemical sensor manifests intriguing application with good sensitivity, selectivity and reproducibility towards the EP, L-Tyr detection. The practical analytical utility provides great promise by selective measurements in tea, and chicken extract which has a promising future for biological and healthcare applications.

KEYWORDS:

Bovine serum albumin; Epinephrine; Irradiated Ppy; L-Tyrosine; Square wave voltammetry

PMID:
29499559
DOI:
10.1016/j.bios.2018.02.044
[Indexed for MEDLINE]

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