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Int J Syst Evol Microbiol. 2018 Apr;68(4):1327-1332. doi: 10.1099/ijsem.0.002676. Epub 2018 Mar 2.

Amnibacterium endophyticum sp. nov., an endophytic actinobacterium isolated from Aegiceras corniculatum.

Li FN1, Tuo L2, Lee SM3, Jin T4,5, Liao S4,5,6, Li W7, Yan X7, Sun CH1.

Author information

1
1​Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
2
2​Research Center for Medicine and Biology, Zunyi Medical University, Zunyi 563003, PR China.
3
3​State Key Laboratory of Quality Research of Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, PR China.
4
4​BGI-Shenzhen, Beishan, Industrial Zone, Shenzhen 518083, PR China.
5
5​China National GeneBank, BGI-Shenzhen, Jinsha Road, Shenzhen 518120, PR China.
6
6​BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, PR China.
7
7​China Pharmaceutical University, Nanjing 210009, PR China.

Abstract

A Gram-stain-positive, aerobic, non-motile, non-spore-forming and short-rod-shaped actinobacterium, designated strain 1T4Z-3T, was isolated from a piece of surface-sterilized branch of Aegiceras corniculatum collected from the Cotai Ecological Zones in Macao, China. Comparative 16S rRNA gene sequence analysis showed that strain 1T4Z-3T was clearly affiliated to the genus Amnibacterium and exhibited 97.9 % gene sequence similarity to Amnibacterium kyonggiense JCM 16463T, 97.3 % gene sequence similarity to Amnibacterium soli JCM 19015T and less than 96.4 % gene sequence similarities to other genera of the family Microbacteriaceae. Strain 1T4Z-3T had L-2,4-diaminobutyric acid as the diagnostic cell-wall diamino acid. The major fatty acids (>10 % of total fatty acids) were iso-C16 : 0 (46.6 %) and anteiso-C15 : 0 (27.3 %). The predominant menaquinones of strain 1T4Z-3T were MK-11 (81.4 %) and MK-12 (14.1 %). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, six unidentified glycolipids, four unidentified phospholipids and two unidentified lipids. The DNA G+C content of strain 1T4Z-3T was 71.4 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic features, strain 1T4Z-3T is considered to represent a novel species of the genus Amnibacterium, for which the name Amnibacterium endophyticum sp. nov. is proposed. The type strain of Amnibacterium endophyticum is 1T4Z-3T (=KCTC 39983T=CGMCC 1.16066T).

KEYWORDS:

Aegiceras corniculatum; Amnibacterium endophyticum; Strain 1T4Z-3T; endophytic actinobacterium

PMID:
29498617
DOI:
10.1099/ijsem.0.002676
[Indexed for MEDLINE]

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