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Front Neurosci. 2018 Feb 15;12:77. doi: 10.3389/fnins.2018.00077. eCollection 2018.

Diffusion Basis Spectrum and Diffusion Tensor Imaging Detect Hippocampal Inflammation and Dendritic Injury in a Virus-Induced Mouse Model of Epilepsy.

Author information

1
Department of Radiology, the First Affiliated Hospital, Nanchang University, Jiangxi, China.
2
Department of Radiology, Washington University in St. Louis, St. Louis, MO, United States.
3
Department of Pathology, University of Utah, Salt Lake City, UT, United States.
4
Department of Chemistry, Washington University in St. Louis, St. Louis, MO, United States.
5
Department of Biomedical Engineering, Washington University, St. Louis, MO, United States.
6
Department of Biostatistics, Washington University in St. Louis, St. Louis, MO, United States.
7
Hope Center for Neurological Disorders, Washington University School of Medicine, Washington University in St. Louis, St. Louis, MO, United States.

Abstract

Hippocampal CA1 inflammation and dendritic loss are common in epilepsy. Quantitative detection of coexisting brain inflammation and injury could be beneficial in monitoring disease progression and assessing therapeutic efficacy. In this work, we used conventional diffusion tensor imaging (DTI, known to detect axonal injury and demyelination) and a novel diffusion basis spectrum imaging (DBSI, known to detect axonal injury, demyelination, and inflammation) to detect hippocampal CA1 lesions resulting from neuronal dendritic injury/loss and concomitant inflammation in Theiler's murine encephalomyelitis virus (TMEV)-induced seizure mice. Following the cross-sectional ex vivo diffusion magnetic resonance imaging measurements, immunohistochemistry was performed to validate DTI and DBSI findings. Both DTI and DBSI detected immunohistochemistry-confirmed dendritic injury in the hippocampal CA1 region. Additionally, DBSI-derived restricted isotropic diffusion tensor fraction correlated with 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI)-positive nucleus counts, and DBSI-derived fiber fraction correlated with dendrite density assessed by microtubule-associated protein 2 staining. DTI-derived fractional anisotropy (FA) correlated with dendrite density and negatively correlated with DAPI-positive nucleus counts. Although both DTI and DBSI detected hippocampal injury/inflammation, DTI-FA was less specific than DBSI-derived pathological metrics for hippocampal CA1 dendritic injury and inflammation in TMEV-induced seizure mice.

KEYWORDS:

Theiler's murine encephalomyelitis virus; dendrite injury; diffusion basis spectrum imaging; diffusion tensor imaging; hippocampal CA1 region; inflammation; magnetic resonance imaging; seizures

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