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Biol Chem. 2018 Sep 25;399(9):1079-1084. doi: 10.1515/hsz-2018-0108.

Kallikrein-related peptidase 14 is the second KLK protease targeted by the serpin vaspin.

Author information

1
Institute of Biochemistry, Faculty of Life Sciences, Leipzig University, BrĂ¼derstrasse 34, D-04103 Leipzig, Germany.
2
Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, Leipzig University, D-04103 Leipzig, Germany.

Abstract

Kallikrein-related peptidases KLK5, KLK7 and KLK14 are important proteases in skin desquamation and aberrant KLK activity is associated with inflammatory skin diseases such as Netherton syndrome but also with various serious forms of cancer. Previously, we have identified KLK7 as the first protease target of vaspin (Serpin A12). Here, we report KLK14 as a second KLK protease to be inhibited by vaspin. In conclusion, vaspin represents a multi-specific serpin targeting the kallikrein proteases KLK7 and KLK14, with distinct exosites regulating recognition of these target proteases and opposing effects of heparin binding on the inhibition reaction.

KEYWORDS:

adipokine; diabetes; exosite; inflammation; kallikrein; metabolic syndrome; obesity; serine protease; serpin A12; site-directed mutagenesis; structural biology

PMID:
29494334
DOI:
10.1515/hsz-2018-0108
[Indexed for MEDLINE]

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