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Nat Commun. 2018 Feb 28;9(1):875. doi: 10.1038/s41467-018-03256-2.

Spatial specificity of auxin responses coordinates wood formation.

Author information

1
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.
2
Centre for Organismal Studies (COS), Heidelberg University, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany.
3
Novosibirsk State University, 2 Pirogova Street, Novosibirsk, 630090, Russian Federation.
4
Institute of Cytology and Genetics, 10 Lavrentyeva Avenue, Novosibirsk, 630090, Russian Federation.
5
Department of Agrotechnology and Food Sciences, Subdivision Biochemistry, Wageningen University and Research Center, Stippeneng 4, 6708WE, Wageningen, The Netherlands.
6
Instituto de Biología Molecular y Celular de Plantas (IBMCP-CSIC), Universidad Politecnica de Valencia, Ingeniero Fausto Elio s/n, 46022, Valencia, Spain.
7
Centre for Organismal Studies (COS), Heidelberg University, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany. thomas.greb@cos.uni-heidelberg.de.

Abstract

Spatial organization of signalling events of the phytohormone auxin is fundamental for maintaining a dynamic transition from plant stem cells to differentiated descendants. The cambium, the stem cell niche mediating wood formation, fundamentally depends on auxin signalling but its exact role and spatial organization is obscure. Here we show that, while auxin signalling levels increase in differentiating cambium descendants, a moderate level of signalling in cambial stem cells is essential for cambium activity. We identify the auxin-dependent transcription factor ARF5/MONOPTEROS to cell-autonomously restrict the number of stem cells by directly attenuating the activity of the stem cell-promoting WOX4 gene. In contrast, ARF3 and ARF4 function as cambium activators in a redundant fashion from outside of WOX4-expressing cells. Our results reveal an influence of auxin signalling on distinct cambium features by specific signalling components and allow the conceptual integration of plant stem cell systems with distinct anatomies.

PMID:
29491423
PMCID:
PMC5830446
DOI:
10.1038/s41467-018-03256-2
[Indexed for MEDLINE]
Free PMC Article

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