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Int J Biol Sci. 2018 Jan 11;14(1):78-86. doi: 10.7150/ijbs.22426. eCollection 2018.

Fam60al as a novel factor involved in reprogramming of somatic cell nuclear transfer in zebrafish (Danio rerio).

Hu H1,2, Tao B1,2, Chen J1, Zhu Z1, Hu W1,3.

Author information

1
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
2
University of Chinese Academy of Science, Beijing 100049, China.
3
Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.

Abstract

The main reason for abnormal development of cloned animals or embryos, and inefficient animal cloning, is a poor understanding of the reprogramming mechanism. To better comprehend reprogramming and subsequent generation of pluripotent stem cells, we must investigate factors related to reprogramming of somatic cells as nuclear donors. As we know, fam60al (family with sequence similarity 60, member A, like) is a coding gene only found in zebrafish and frog (Xenopus laevis) among vertebrates. However, until now, its functions have remained unknown. Here, we generated a zebrafish fam60al-/- mutant line using transcription activator-like effector nucleases (TALENs), and found that both nanog and klf4b expression significantly decreased while myca expression significantly increased in fam60al-/- mutant embryos. Concurrently, we also uncovered that in developmentally arrested embryos of somatic cell nuclear transfer, nanog, klf4b and myca expression was down-regulated, accompanying a decrease of fam60al expression. Interestingly, we identified a long noncoding RNA (lncRNA) of fam60al, named fam60al-AS, which negatively regulated fam60al by forming double-stranded RNA (dsRNA). RNase protection assay and real-time PCR confirmed these findings. Taken together, these results suggest that fam60al is a novel factor related to the reprogramming of somatic cell nuclear transfer in zebrafish, which is regulated by its reverse lncRNA.

KEYWORDS:

Fam60al; long noncoding RNA; myca.; nanog; reprogramming; somatic cell nuclear transfer

PMID:
29483827
PMCID:
PMC5821051
DOI:
10.7150/ijbs.22426
[Indexed for MEDLINE]
Free PMC Article

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