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Nat Commun. 2018 Feb 26;9(1):834. doi: 10.1038/s41467-018-03229-5.

Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis.

Author information

1
MRC London Institute of Medical Sciences, Imperial College London, London, W12 0NN, UK.
2
Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA.
3
SPST, Tianjin University, Tianjin, 300072, China.
4
Barts Cancer Institute, London, EC1M 6BQ, UK.
5
The Francis Crick Institute, London, NW1 1AT, UK.
6
MRC London Institute of Medical Sciences, Imperial College London, London, W12 0NN, UK. enrique.martinez-perez@imperial.ac.uk.

Abstract

The formation of haploid gametes from diploid germ cells requires the regulated two-step release of sister chromatid cohesion (SCC) during the meiotic divisions. Here, we show that phosphorylation of cohesin subunit REC-8 by Aurora B promotes SCC release at anaphase I onset in C. elegans oocytes. Aurora B loading to chromatin displaying Haspin-mediated H3 T3 phosphorylation induces spatially restricted REC-8 phosphorylation, preventing full SCC release during anaphase I. H3 T3 phosphorylation is locally antagonized by protein phosphatase 1, which is recruited to chromosomes by HTP-1/2 and LAB-1. Mutating the N terminus of HTP-1 causes ectopic H3 T3 phosphorylation, triggering precocious SCC release without impairing earlier HTP-1 roles in homolog pairing and recombination. CDK-1 exerts temporal regulation of Aurora B recruitment, coupling REC-8 phosphorylation to oocyte maturation. Our findings elucidate a complex regulatory network that uses chromosome axis components, H3 T3 phosphorylation, and cell cycle regulators to ensure accurate chromosome segregation during oogenesis.

PMID:
29483514
PMCID:
PMC5827026
DOI:
10.1038/s41467-018-03229-5
[Indexed for MEDLINE]
Free PMC Article

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