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Microbiome. 2018 Feb 27;6(1):43. doi: 10.1186/s40168-018-0429-0.

A novel affordable reagent for room temperature storage and transport of fecal samples for metagenomic analyses.

Han M1,2, Hao L3,4, Lin Y3,4, Li F3,4, Wang J3,4,5, Yang H3,4,5, Xiao L3,4,6, Kristiansen K3,7, Jia H8,9,10, Li J11,12,13,14.

Author information

1
BGI-Shenzhen, Shenzhen, 518083, China. hanmo@genomics.cn.
2
China National Genebank, BGI-Shenzhen, Shenzhen, 518120, China. hanmo@genomics.cn.
3
BGI-Shenzhen, Shenzhen, 518083, China.
4
China National Genebank, BGI-Shenzhen, Shenzhen, 518120, China.
5
James D. Watson Institute of Genome Sciences, Hangzhou, 310058, China.
6
Shenzhen Engineering Laboratory of Detection and Intervention of Human Intestinal Microbiome, Shenzhen, 518083, China.
7
Department of Biology, Laboratory of Genomics and Molecular Biomedicine, University of Copenhagen, Universitetsparken 13, 2100, Copenhagen, Denmark.
8
BGI-Shenzhen, Shenzhen, 518083, China. jiahuijue@genomics.cn.
9
China National Genebank, BGI-Shenzhen, Shenzhen, 518120, China. jiahuijue@genomics.cn.
10
Shenzhen Key Laboratory of Human commensal microorganisms and Health Research, BGI-Shenzhen, Shenzhen, 518083, China. jiahuijue@genomics.cn.
11
BGI-Shenzhen, Shenzhen, 518083, China. lijunhua@genomics.cn.
12
China National Genebank, BGI-Shenzhen, Shenzhen, 518120, China. lijunhua@genomics.cn.
13
School of Bioscience and Biotechnology, South China University of Technology, Guangzhou, 510006, China. lijunhua@genomics.cn.
14
Shenzhen Key Laboratory of Human commensal microorganisms and Health Research, BGI-Shenzhen, Shenzhen, 518083, China. lijunhua@genomics.cn.

Abstract

BACKGROUND:

The number of large-scale studies on the gut microbiota in human cohorts is rapidly increasing. However, the few and expensive options for storage of fecal samples at room temperature have been an obstacle for large-scale metagenomic studies and the development of clinical/commercial personal metagenomic sequencing.

RESULTS:

In this study, we systematically tested a novel N-octylpyridinium bromide-based fecal sample preservation method and compared it with other currently used storage methods. We found that the N-octylpyridinium bromide-based method enabled preservation of the bacterial composition in fecal samples transported and stored at room temperature for up to at least 14 days.

CONCLUSIONS:

We describe a novel chemical stabilizer that allows cost-effective transportation and storage at room temperature for several days with preservation of bacterial composition. This method will facilitate sample collection even in remote area and also enable transport via normal commercial transportation routes.

KEYWORDS:

Fecal sample; Metagenomic sequencing; N-octylpyridinium bromide; Room temperature storage; Room temperature transport

PMID:
29482661
PMCID:
PMC5828344
DOI:
10.1186/s40168-018-0429-0
[Indexed for MEDLINE]
Free PMC Article

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