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Methods Mol Biol. 2018;1732:477-493. doi: 10.1007/978-1-4939-7598-3_30.

Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis.

Author information

1
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
2
Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada. morgan.fullerton@uottawa.ca.

Abstract

Macrophages are a driving force in the development and progression of atherosclerosis, a chronic condition that can lead to cardiovascular disease. In this chapter we describe methods that monitor macrophage cholesterol homeostasis such as cholesterol synthesis, uptake, and efflux, all with the use of AMPK activators and potential genetic models that could help shed light on the role of this metabolic regulator in atherosclerosis and other chronic diseases.

KEYWORDS:

AMP-activated protein kinase; Atherosclerosis; Cholesterol homeostasis; Macrophages; Reverse cholesterol transport

PMID:
29480494
DOI:
10.1007/978-1-4939-7598-3_30
[Indexed for MEDLINE]

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