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Food Chem. 2018 Jun 30;252:40-48. doi: 10.1016/j.foodchem.2018.01.085. Epub 2018 Jan 12.

Simultaneous determination of clanobutin, dichlorvos, and naftazone in pork, beef, chicken, milk, and egg using liquid chromatography-tandem mass spectrometry.

Author information

1
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
2
Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt. Electronic address: amabdelaty@konkuk.ac.kr.
3
Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkey.
4
Department of Pharmacology, College of Medicine, Chung-Ang University, 221, Heuksuk-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
5
Institute of Quality Standard and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China.
6
Natural Products Chemistry Laboratory, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, Republic of Korea.
7
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea. Electronic address: hshin@konkuk.ac.kr.

Abstract

A chromatographic method involving a single run was validated for the quantification of clanobutin, dichlorvos, and naftazone in products of animal origin. Pork, beef, chicken, milk, and egg samples were extracted with a solution of 0.1% formic acid in acetonitrile, defatted with n-hexane, centrifuged, and filtered prior to analysis using liquid chromatography-tandem mass spectrometry (LC-/MS/MS). The analytes were separated on a C18 column using a solution of 0.1% formic acid and 10 mM ammonium formate (A) and acetonitrile (B) as the mobile phase. A good linearity over 5-50 ng/g concentration range was obtained with coefficients of determination (R2) ≥ 0.9807. The intra- and interday accuracy (recovery %) calculated from 3 fortification levels (5, 10, and 20 ng/g) were 73.2-108.1% and 71.4-109.8%, and the precisions (expressed relative standard deviations (RSDs)) were 0.9-12.9% and 1.8-10.6%, respectively, for the 3 tested analytes in animal originated foods. The limits of quantification (LOQs) ranged between 0.1 and 1 ng/g, thus enabling the quantification of residual levels below the uniform maximum residue limit (MRL) of 0.01 mg/kg set for compounds having no MRL. The designated methodology was successfully applied to monitor various samples collected from Seoul; the tested analytes were not quantified in any of the market samples. Conclusively, the developed method is simple, sensitive, and accurate, and could be used for the detection of pharmaceuticals in various animal food matrices with variable protein and fat contents.

KEYWORDS:

Animal-based food products; Clanobutin; Determination; Dichlorvos; Naftazone; Residue analysis; Simultaneous

PMID:
29478562
DOI:
10.1016/j.foodchem.2018.01.085
[Indexed for MEDLINE]

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