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Cell Motil Cytoskeleton. 1986;6(5):502-9.

Rhamnolipid from Pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes.

Abstract

Isolated ciliary axonemes from pig trachea were exposed to increasing concentrations of purified Pseudomonas aeruginosa rhamnolipid. This is a defined ciliary system allowing observation of direct impairment of functional axonemes. Axonemal motility and ATPase activity were decreased in proportion to rhamnolipid concentrations. ATPase-associated proteins observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dynein arms seen in ultra-structural cross sections progressively disappeared from axonemes with exposure to rhamnolipid. These four independent measures establish that the rhamnolipid removes the ATPase-containing outer dynein arms from the ciliary axoneme, thereby rendering the axoneme immotile.

PMID:
2947698
DOI:
10.1002/cm.970060509
[Indexed for MEDLINE]

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