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Methods Mol Biol. 2018;1719:311-318. doi: 10.1007/978-1-4939-7537-2_20.

Methodology for Urine Peptidome Analysis Based on Nano-HPLC Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.

Author information

1
Moscow Institute of Physics and Technology, Moscow, Russia. alex.kononikhin@gmail.com.
2
V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia. alex.kononikhin@gmail.com.
3
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia. alex.kononikhin@gmail.com.
4
Moscow Institute of Physics and Technology, Moscow, Russia.
5
V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
6
Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
7
V.I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
8
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
9
Moscow Institute of Physics and Technology, Moscow, Russia. ennikolaev@gmail.com.
10
V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia. ennikolaev@gmail.com.
11
Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia. ennikolaev@gmail.com.
12
Skolkovo Institute of Science and Technology, Skolkovo, Moscow Region, Russia. ennikolaev@gmail.com.

Abstract

Urine is a sample of choice for noninvasive biomarkers search because it is easily available in large amounts and its molecular composition provides information on processes in the organism. The high potential of urine peptidomics has been demonstrated for clinical purpose. Several mass spectrometry based approaches have been successfully applied for urine peptidome analysis and potential biomarkers search. Summarizing literature data and our own experience we developed a protocol for comprehensive urine peptidome analysis. The technology includes several stages and consists of urine sample preparation by size exclusion chromatography and identification of featured peptides by nano-HPLC coupled to Fourier transform ion cyclotron resonance mass spectrometry, semiquantitative and statistical data analysis.

KEYWORDS:

FTICR MS; LC-MS/MS; Size exclusion chromatography; Urine peptidome

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