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In Vivo. 2018 Mar-Apr;32(2):241-247.

Plasma Electrolytic Oxidation of Titanium Implant Surfaces: Microgroove-Structures Improve Cellular Adhesion and Viability.

Author information

1
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
2
Meotec GmbH & Co. KG, Aachen, Germany.
3
Department of Oral & Maxillofacial Surgery, Charité - Universitätsmedizin Berlin, Berlin, Germany.
4
Fresenius University Medical School, Idstein, Germany.
5
Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany ol.jung@uke.de.

Abstract

BACKGROUND/AIM:

Plasma electrolytic oxidation (PEO) is an established electrochemical treatment technique that can be used for surface modifications of metal implants. In this study we to treated titanium implants with PEO, to examine the resulting microstructure and to characterize adhesion and viability of cells on the treated surfaces. Our aim was to identify an optimal surface-modification for titanium implants in order to improve soft-tissue integration.

MATERIALS AND METHODS:

Three surface-variants were generated on titanium alloy Ti6Al4V by PEO-treatment. The elemental composition and the microstructures of the surfaces were characterized using energy dispersive X-ray spectroscopy, scanning electron microscopy and profilometry. In vitro cytocompatibility of the surfaces was assessed by seeding L929 fibroblasts onto them and measuring the adhesion, viability and cytotoxicity of cells by means of live/dead staining, XTT assay and LDH assay.

RESULTS:

Electron microscopy and profilometry revealed that the PEO-surface variants differed largely in microstructure/topography, porosity and roughness from the untreated control material as well as from one another. Roughness was generally increased after PEO-treatment. In vitro, PEO-treatment led to improved cellular adhesion and viability of cells accompanied by decreased cytotoxicity.

CONCLUSION:

PEO-treatment provides a promising strategy to improve the integration of titanium implants with surrounding tissues.

KEYWORDS:

Plasma electrolytic oxidation (PEO); cell adhesion; in vitro cytocompatibility; surface topography

PMID:
29475905
PMCID:
PMC5905190
DOI:
10.21873/invivo.11230
[Indexed for MEDLINE]
Free PMC Article

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