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Cell. 2018 Feb 22;172(5):910-923.e16. doi: 10.1016/j.cell.2018.01.035.

Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
2
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
3
Broad Institute of MIT and Harvard, Cambridge, MA 02136, USA.
4
Department of Biological Sciences, Columbia University, New York, NY 10027, USA. Electronic address: mj2794@columbia.edu.
5
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: gabrar@berkeley.edu.

Abstract

To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous genome-wide measurements of mRNA, translation, and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti-correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases, or over 8% of all measured genes, involves temporally regulated switching between production of a canonical, translatable transcript and a 5' extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a single transcription factor can coordinately activate and repress protein synthesis for distinct sets of genes. The distinction is not based on whether or not an mRNA is induced but rather on the type of transcript produced.

KEYWORDS:

LUTI; coordination; differentiation; gene expression; isoform; meiosis; ribosome profiling; transcription factor; translation; uORF

PMID:
29474919
PMCID:
PMC5826577
DOI:
10.1016/j.cell.2018.01.035
[Indexed for MEDLINE]
Free PMC Article

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