Send to

Choose Destination
J Biomed Opt. 2018 Feb;23(2):1-4. doi: 10.1117/1.JBO.23.2.020504.

Benchtop and animal validation of a portable fluorescence microscopic imaging system for potential use in cholecystectomy.

Author information

University of Science and Technology of China, Department of Precision Machinery and Precision Instr, China.
Anhui Provincial Hospital, Department of General Surgery, Hefei, Anhui, China.
Ohio State University, Department of Biomedical Engineering, Columbus, Ohio, United States.


We propose a portable fluorescence microscopic imaging system (PFMS) for intraoperative display of biliary structure and prevention of iatrogenic injuries during cholecystectomy. The system consists of a light source module, a camera module, and a Raspberry Pi computer with an LCD. Indocyanine green (ICG) is used as a fluorescent contrast agent for experimental validation of the system. Fluorescence intensities of the ICG aqueous solution at different concentration levels are acquired by our PFMS and compared with those of a commercial Xenogen IVIS system. We study the fluorescence detection depth by superposing different thicknesses of chicken breast on an ICG-loaded agar phantom. We verify the technical feasibility for identifying potential iatrogenic injury in cholecystectomy using a rat model in vivo. The proposed PFMS system is portable, inexpensive, and suitable for deployment in resource-limited settings.


biliary injuries; cholecystectomy; fluorescence microscopic imaging; image-guided surgery


Supplemental Content

Loading ...
Support Center