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J Biomed Opt. 2018 Feb;23(2):1-4. doi: 10.1117/1.JBO.23.2.020504.

Benchtop and animal validation of a portable fluorescence microscopic imaging system for potential use in cholecystectomy.

Author information

1
University of Science and Technology of China, Department of Precision Machinery and Precision Instr, China.
2
Anhui Provincial Hospital, Department of General Surgery, Hefei, Anhui, China.
3
Ohio State University, Department of Biomedical Engineering, Columbus, Ohio, United States.

Abstract

We propose a portable fluorescence microscopic imaging system (PFMS) for intraoperative display of biliary structure and prevention of iatrogenic injuries during cholecystectomy. The system consists of a light source module, a camera module, and a Raspberry Pi computer with an LCD. Indocyanine green (ICG) is used as a fluorescent contrast agent for experimental validation of the system. Fluorescence intensities of the ICG aqueous solution at different concentration levels are acquired by our PFMS and compared with those of a commercial Xenogen IVIS system. We study the fluorescence detection depth by superposing different thicknesses of chicken breast on an ICG-loaded agar phantom. We verify the technical feasibility for identifying potential iatrogenic injury in cholecystectomy using a rat model in vivo. The proposed PFMS system is portable, inexpensive, and suitable for deployment in resource-limited settings.

KEYWORDS:

biliary injuries; cholecystectomy; fluorescence microscopic imaging; image-guided surgery

PMID:
29473349
DOI:
10.1117/1.JBO.23.2.020504

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